Genotyping of Acinetobacter baumannii by using BOX Method

Fifty isolates of Acinetobacter baumannii have been collected from Iraqi patients with different infection included wounds and burns infection during the period from July to October 2018. After bacteria isolation and identification, 20 isolates of Acinetobacter baumannii were confirmed. The BOX-PCR method was used to detect the relationship among these bacteria isolates, the fingerprinting patterns of the isolates were shown 16 bands on gel electrophoresis, with molecular weight ranging between (100–3000 bp) among the 20 Acinetobacter baumannii isolates. Genetic relationship of different isolates of Acinetobacter baumannii was done by using BOX-PCR method and Dendogram analysis. The results showed the genetic relationship between Acinetobacter baumannii 1 clones, while 18 isolates contained different genotyping. In conclusion, the study found that BOX method is reproducible, easy, fast and cost effectiveness method to study the typing between Acinetobacter baumannii isolates.