Determination of Lethal Effectiveness of (Thymus Vulgaris) Oil Against Major Oral Pathogen Using Different Assay: In Vitro Study

The aim of the research was to determine the lethal effectiveness of Thymus vulgaris oil against some oral pathogens (Staph. aureus, and Strept. mutans). The microbial activity was determined for different concentration(10%&20%) of oil of Thymus vulgaris and tested on sixty nine isolates of Staph. aureus, and Strept. mutans which have been isolated from different oral samples (gingival, and dental caries swabs) for students of the department of dental prevention of technical medical institute of Baghdad. The antibacterial activity of different concentrations (10% & 20%) of the oil of Thymus vulgaris were examined against two oral pathogenes. The essential oil mixed with dimethylsulfoxide (DMSO). Sterilized discs (Whatmann NO 6) were impregnate with 15 μl of different concentrations of the oil and DMSO Then placed on the surface of the dishes planted the two bacteria The antibacterial effects at 10% & 20% concentration of oil using the disc diffusion method against twenty five isolates of Staphylococcus aureus and forty four isolates of Streptococcus mutans showed that oil of Thymus vulgaris significantly inhibited the activity of S.aurus and S. mutans growth in 2% concentration by disc diffusion and well diffusion with inhibition zones of, 12.4±0.91 and13.9±0.22 respectively.