Influence of TLR4 Polymorphisms on Susceptibility to UTI Caused by Gram Negative Bacteria in Pregnant Women

Urinary tract infections (UTI) are serious health problems affecting millions of people each year. This study aimed to investigate the effect of TLR4 gene polymorphisms on the pregnant women susceptibility to urinary tract infection caused by Gram negative bacteria. a total of 15 clinically confirmed UTI pregnant women and 35 healthy control were enrolled in this study. Urine and blood samples were collected in Babylon teaching hospital for gynecology and pediatrics in Babylon province, Iraq during the period from January 2018 to February 2019. Urinary sample isolates were identified by traditional methods. DNA was extracted using blood samples. TLR4 gene amplification and SNP genotyping was done using PCR-RFLP. Cytokine profile was assessed using ELISA technique. The present study showed 12 (80%) isolates was Escherichia coli; 2 (13.3%) was Klebsellapneumonia and only one isolate (6.7%) was Pseudomonas aerogenosa. the results showed the presence of homozygous variant in 11 patient from the study group at site 299 (A/G).Two homozygous variantand 8 heterozygous variant at site 399 (C/T). Serum IL17 results showed a significant difference (LSD value=26.3pg/ml) between patients and controls. Serum TNF-αresults showed a significant difference (LSD value=9.8pg/ml) between patients and controls.