Effect of Oxygenated Water Alone or with Vaccines and Antibiotics on Immune Response Against Viral Diseases and Intestinal Microflora Community in Broiler Chickens

This study was conducted to investigate the effect of providing oxygenated water with or without vaccines and antibiotic on immune response against Newcastle disease (ND), Infectious Bronchitis disease (IB), Bursal disease (BD), total aerial bacteria, coliform bacteria and Lactobacilli bacteria in broiler chicks. A total of 160 broiler chicks (Ross 308) one day old were randomly distributed to 4 groups each with 4 replicates (10 birds/replicate) as follow: group 1 (C-) tap water without vaccines and antibiotic, group 2 (C +) tap water with vaccines and antibiotic, group 3 (OW-) oxygenated water without vaccines and antibiotic, group 4 (OW +) oxygenated water with vaccines and antibiotic. Blood samples were collected two times on days 21 and 42 of age to measure antibiotic titers against ND, IB and BD diseases. Microbial count for total aerobic bacteria, coliform bacteria and lactobacilli bacteria were evaluated at the end of the study (42 days old). The results indicated that the antibody titers against ND, IB, and BD disease was significantly higher (p<0.05) in group OW +(3903±80.44) and (4731.9±71.6) followed by group C +(2123±241.1) and (36101±4.7), group OW-(1928±72.34) and (2128±32.7) and finally group C-(924±11.2) and (605±10.3) for the age group 21days and 42 days respectively. There were no differences among experimental groups in total aerobic bacteria. The lactobacilli bacteria significantly (p< 0.05)increased in the different experimental groups as follow OW +(7.01±0.33), OW-(6.01–0.52), C-(5.63–0.31), while the coliform bacteria values were significant lower (p < 0.05) in WO+, C +, OW-and C-and it was (4.74±0.33), (5.67±0.81), (6.7±40.46) and (7.09±0.39) respectively. These results indicate that providing oxygenated water with vaccines and antibodies may be recommended as a cofactor to improve immune response against common viral diseases and pathogenic bacteria in broilers.