Indian Journal of Forensic Medicine & Toxicology

to determine the effect of Nigella sativa ethanol extract on serum sFlt-1 levels and expression of renal ET-1 mice model of preeclampsia.

This research was a laboratory experimental research with post test only control group design. This study used 30 BALB/C mice which were divided into 6 groups, namely negative control which was pregnant mice injected with serum of normal pregnant women, positive control was preeclampsia model mice, and treatment groups 1, 2, 3 and 4 were mouse models of preeclampsia get 500 mg, 1000 mg, 1500mg and 2000mg/kgBB/day ethanol extract of Nigella sativa for 5 days. Statistical analysis using ANOVA.

Mean renal ET-1 expression in mice preeclampsia model in treatment groups were 500mg, 1000mg, 1500mg and 2000mg (9.6+1.8%, 6.8+1.3%, 4.6+1.5% dan 5.4+1.5%) showed significant differences (p <0.05) and found a decrease in renal ET-1 expression with increasing dose. Same like that, mean expression of eNOS kidney showed significant differences in the treatment group dose 500mg, 1000mg, 1500mg and 2000mg (56.3±3.2%, 63.8±3.3%, 78.0±5.4%, 66.9±1.9%).

The administration of ethanol extract of black cumin seeds (Nigella sativa) decrease renal ET-1 expression and increase renal eNOS expression mice preeclampsia model and the effect was dose dependent.