Thin layer chromatographic and spot test detection of carbosulfan by alkaline fast Blue-B reagent

Thin layer chromatographic and spot test detection of carbosulfan, a carbamate pesticide by alkaline fast blue-B reagent is described. Carbosulfan on alkaline hydrolysis forms a phenolic product which further condenses with fast blue-B and forms orange coloured complex on silica gel-G TLC plate. Sensitivity of this reagent to detect the carbosulfan was found to be 5μg. The method can be applied to detect this carbamate in various samples including forensic samples.