*Department of Oral Pathology, Maharishi Markendeshwar, College of Dental Studies and Research, Ambala, Haryana-133 203
**Department of Biotechnology, Institute of Technology and Science, Delhi-Meerut Road, Murad Nagar 201 206, UP
1Address for correspondence: Chatterjee S M.D.S. Assistant Professor Department of Oral Pathology, Maharishi Markendeshwar – College of Dental Studies and Research, Ambala (Haryana)-Email id: shailjachatterjee@gmail.com
DNA is an important evidentiary tool for victim identification and verification in forensic science. The role of forensic odontologist is becoming increasingly important for the same purpose as the rate of mass disaster situations and terror strikes have become a day-to-day reality.
This study paper deals with demonstration of viability of DNA harvested from teeth subjected to increasingly elevated temperatures.
DNA isolation from intact tooth specimens subjected to elevated heat-treatment was done using agarose gel electrophoresis technique. DNA bands were visualized under UV light.
The presence of viable DNA was demonstrable from all the specimens.
Obtainment of identifiable DNA is a strong possibility in teeth that have been subjected to increasingly higher temperatures, therefore, increasing the scope of a single tooth specimen as a valuable source for DNA.
DNA, agarose gel electrophoresis, forensic odontologist, teeth. DNA extraction from heat-treated dental pulp using agarose gel electrophoresis