1M.Sc Student, Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri
2Junior Research Fellow, Central Research Laboratory, A B Shetty Memorial Institute of Dental Sciences, Nitte University
3Professor, Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri
4Assistant Professor, Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri, Karnataka
*Corresponding author: Chandrashekar G Joshi, Assistant Professor, Department of Biochemistry, Mangalore University. Phone: 9448446641. E mail: josheejoshee@gmail.com
Online published on 23 July, 2013.
The aim of present study is to evaluate cytotoxic and genotoxic activity of Moullava spicata (Dalz.) Nicolson.
The aqueous extract of this plant subjected to genotoxic and cytotoxic study using yeast viability test, hemolytic assay and Allium test.
The extract showed dose dependent effect on yeast viability and hemolysis of erythrocytes. The Allium test showed significant reduction of root length (p > 0.01) and number (p > 0.001) indicated cytotoxic effect of plant extract. Microscopic observations showed cytotoxic effects such as ghost cell, membrane damage or rupture, apoptotic bodies and the extent damage was increased significantly after 96 hours treatment.
The study showed that the leaf extract of the M. spicata contains various biologically active compounds and possess cytotoxic effects.
Moullava spicata (Dalz.) Nicolson, Allium test, Hemolysis, Cytotoxicity
