Effects of He-Ne laser and Enhanced Ultravlolet-B Radiation on Lamin B of Wheat

The 7d seedlings of wheat (‘ML 7113′) were exposed to He-Ne laser irradiation (L) with 5mW·mm-2 power density after enhanced UV-B radiation (10.08KJ·m-2·d-1). Total protein were extracted in each treatment group. The total protein were assayed by SDS-PAGE and identified by Western Blot. And analyses the influence of enhanced UV-B radiation-induced wheat leaves lamin B damage repair effects by He-Ne laser. The results shows that enhanced UV-B radiation can damage the lamin B in the seedlings. It may have certain role for repairing the damaged lamin B irradiated by He-Ne laser in the wheat seedlings. In order to futher investigate the effects of UV-B radiation on the relationships between laminB arrangements and ‘Partition-bundle division'the dynamic variation of laminB in wheat root-tip cells was studied with confocal laser scanning microscopy(CLSM). The results shows that laminB arrays at the periphery of the nucles.