1Department of Horticulture Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
3Department of Genetics and Plant Breeding, Bu-Ali Sina University, Tehran, Iran
*Email for correspondence: moosaviamir900@gmail.com
Online published on 3 February, 2021.
To illustrate the genetic variation in two in vitro culture samples of the medicinal plant Hyoscyamus niger including new (one-month old) and old (one-year old) calli versus fresh leaves, the inter simple sequence repeat (ISSR) marker was used. By using 10 primers, 6.49 per cent polymorphism was found in three studied populations. The frequency of somatic variation tended to increase with the increasing subculture time. Analysis of molecular variance (AMOVA) revealed the occurrence of 68 per cent of the variation among populations. UPGMA tree discriminated samples in two major groups and principal component analysis (PCoA) confirmed clustering. The first major cluster included old callus and the second involved new callus and fresh leaves. The high potential of H niger along with its high growth rate and abundance of secondary metabolites can make it a suitable species for plant breeding programmes to introduce a cultivar along with the programme’s goals.
Hyoscyamus niger, Somaclonal variation, ISSR marker, Successive subculture, Cluster analysis
