International Journal of Farm Sciences

The present investigation was carried out on Jasminum sambac (L) Aiton belonging to family Oleaceae. The different vegetative parts viz nodal explants, shoot apices, stems and leaves were used to standardize the protocol in vitro. The explants were excised from field grown mature plants and thereafter planted on variously supplemented Murashige and Skoog's (MS) medium for multiple shoot proliferation and callus induction. Early bud break (25–26 days) and maximum percentage of bud break (83.7) was observed in J sambac when MS medium was supplemented with BAP (2.0 mg/l), Kn (1.0 mg/l) and Ads (50 mg/l). Maximum percentage of multiple shoots was observed on MS medium fortified with 2 mg/l BAP, 1.0 mg/l Kn and 50 mg/l Ads. Callus induction from leaf and meristem explants was achieved using different concentrations of auxins and cytokinins. Maximum percentage of cultures showing callus proliferation from leaf explants was observed in the medium containing MS basal salts supplemented with 3.0 mg/l BAP and 2.0 mg/l IAA in leaf explants. The percentage of cultures showing callus was similar in the medium containing MS basal salts supplemented with 5.0 mg/l BAP and 2.0 mg/l NAA in leaf explants. However white and friable callus was obtained in the medium containing MS basal salts supplemented with 2.0 mg/l BAP, 2.0 mg/l Kn and 2.0 mg/l IAA from meristem explants.