1Department of Fruit Science, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan-173230, HP, India
Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan-173230, HP, India
*Email forcorrespondence: shilpachaudhary05@gmail.com
Online published on 25 July, 2018.
In present study among different concentrations of PCR reaction mixture components used, 1X buffer A (1.5 mM MgCl2), 0.7 mM MgCl2, 1 mM dNTP, I μM primer (each Forward and Reverse), 1 U taq DNA polymerase and 30 ng DNA gave best results and were further used to validate 43 EST-SSRs among six genotypes of peach of which 38 primers produced amplification consisting of 18 monomorphic and 20 polymorphic primers. Total number of amplified bands was 75 out of which 51 were polymorphic representing 68.00 per cent polymorphism. High level of polymorphism revealed using EST-SSRs confirms their promising use in molecular studies.
EST-SSRs, peach, standardization, polymorphism
