Development of multiplex PCR for the simultaneous detection of Trypanosoma evansi, Babesia canis and Hepatozoan canis in dogs

Since co-infections with canine tick borne pathogens are common there is greater risk for multiple infections which may necessitate different clinical management strategies. In the present study a multiplex polymerase chain reaction (mPCR) by using the species-specific primers was developed for the simultaneous detection of the most pathogenic haemoparasites, Trypanosoma evansi, Babesia canis and Hepatozoan canis from the blood of naturally-infected dogs so as to enable early diagnosis and treatment.