Synchronization response and conception rate with Ovsynch protocol in buffalo under field condition

A total 30 cyclic buffaloes were selected from village Lakhani District Bhandara (MS) and divided into three equal groups. The selected buffaloes were treated with 2 ml normal saline i/m for 1^st group, PGF₂α (Cloprostinol 500 mcg) 11 days apart randomly and AI was done at observed oestrus for 2^nd group and GPG - Ovsynch protocol (GnRH + PG + GnRH on day 0, 7 & 9, respectively) for 3^rd group with fixed time AI 18–24 hrs later. The overall oestrus response was 20, 50 and 80% in Group I, II and III, respectively. The average duration for onset of oestrus was 177.5 ± 24.50 hrs, 60.80 ± 2.86 hrs and 70.62 ± 14.82 hrs in Group I, II and III, respectively. The average length of oestrus was 16 ± 2.00 hrs, 14.2 ± 0.66 hrs and 14.87 ± 0.87 hrs in Group I, II and III, respectively. The corresponding average score for intensity of oestrus was 43 ± 1.00, 35.00 ± 2.16 and 39.37 ± 2.14 in three groups. The conception rate obtained was 10, 30 and 40% in Group I, Group II and Group III, respectively. The ovsynch protocol was better for improving conception rate in field buffaloes.