Evaluation of non-oxidative immunodefense mechanism of polymorphonuclear cells of goat (Capra hircus)

In this study, the presence of PMN granular enzymes and their antimicrobial potency was tested in goat, and comparative efficacy of three different methods (TritonX-100 with and without acetic acid and 10% acetic acid) for extracting enzymes was also determined. Both the TritonX-100 treated homogenates with and without acetic acid yielded considerable myeloperoxidase, alkaline phosphatase, elastase and acid phosphatase activity. TritonX-1 00 alone was found to be effective for assay of most of the granular enzymes as it produced significant level (P<0.05) of activity compared to other two fractions, whereas 10% acetic acid yielded maximum level of lysozyme and elastase activity. Further, all the three extracts revealed antibacterial efficacy against S. aureus and E. coli strains, but only 10% acetic acid treated homogenate showed antifungal property against C. albican.