Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, India
The present study was carried out to reveal haplotype and phylogenetic analysis of OLR1 gene in Mehsana and Banni breeds of buffalo. Thirty two nucletotide sequences generated from our previous study (Jawale, 2009) were used to reveal the haplotypes in OLR1 (Intron I) gene in the population of Mehsana and Banni buffalo. Two haplotype groups each contaning a set of three Single Nucleotide Polymorphisms were formed, viz. A and B. Four haplotypes were observed in the haplotype block A, viz. GAT, AGT, AAC and AAT with frequencies 0.031, 0.031, 0.031 and 0.906, respectively. Again four haplotypes were observed in haplotype block B, viz. CCC, TTC, TCG and TCC with similar frequencies of 0.031, 0.031, 0.031 and 0.906, respectively. Phylogenetic analysis of these 32 sequences with the same region of Bos taurus distributed the sequences into three clusters based on the homology between them. Cluster I contained twenty eight sequences having 100% homology with each other. Cluster II contained six sequences each having a Single Nucleotide Polymorphism. Cluster III contained the reference sequence of Bos taurus which remained as an outgroup on account of the difference in bases at many loci in comparison to Bubalus bubalis.
Phylogenetics, Haplotypes, OLR1, Bubalus bubalis
