Department of Veterinary Medicine, College of Veterinary Sci. & A. H., Mhow, M.P
Online published on 31 October, 2013.
In the present study efficacy of HSOAV in buffalo calves was ascertained by detection of protective antibody titre using ELISA test and host challenge by inoculating 1ml subcutaneously (s/c) of 1:10 dilution in nutrient broth of 18 hrs old pure culture of P. multocida, P-52 strain. The analysis of variance for antibody titre HSOAV revealed highly significant (P< 0.01) effect of time intervals. Antibody titre showed increasing trend at different time intervals throughout the study period. HSOAV can protect the animals successfully for more than 6 months.
HS, HSOAV, ELISA, Male Buffalo Calves
