Indian Journal of Field Veterinarians (The)
Open Access
  • Year: 2012
  • Volume: 7
  • Issue: 3

Comparison of serological methods for the detection of Brucella Abortus antibodies in sera from infected bovines

  • Author:
  • A. S. Aher, S. P. Londhe, P. P. Mhase, A. S. Bannalikar, V. D. Dighe
  • Total Page Count: 3
  • Page Number: 14 to 16

Department of Microbiology, Bombay Veterinary College, Parel, Mumbai-400 012

Online published on 19 December, 2013.

Abstract

The efficacy of rose Bengal plate test (RBPT), standard tube agglutination test (STAT) and indirect enzyme linked immunosorbent assay (i-ELISA) in detection of bovine brucellosis was evaluated. The serum samples of 74 animals (68 cattle and 6 buffaloes) from different locations in Maharashtra were investigated for presence of Brucella antibodies by RBPT, STAT and i-ELISA. Overall observation of results of three serological tests employed in the present investigation for detection of bovine brucellosis suggests superiority of i-ELISA (56.75%) over RBPT (54.05%) and that of RBPT over STAT (47.29%) in detecting the Brucella antibodies.

Keywords

Brucella, i-ELISA, STAT, RBPT