Effect of Sodium citrate egg yolk (EYC) and Coconut milk extender (CME) on seminal characteristics and artificial insemination in german shepherd dogs

The aim of present study was to evaluate the seminal characteristics as an index for predicting fertilizing capacity and compare preservability of German Shepherd dog semen in two dilutors i.e. Sodium citrate egg yolk (EYC) and Coconut milk extender (CME). Samples were preserved at 50C in sodium citrate egg yolk (EYC) and at room temperature in coconut milk extender (CME) upto 72 hrs to see the effect of dilutors on the individual motility, percentage of live spermatozoa and morphological abnormalities. The average morphological abnormalities recorded in EYC were 4.54±0.10, 8.18±0.03, 12±0.15 and 14.96±0.26% and in CME dilutor were 6.98±0.25, 11.35±0.33, 14.87±0.35 and 18.56±0.38% at 0,24,48, and 72 hrs, respectively. Analysis of variance revealed that there was a significant (P<0.05) difference between hours within dilutor and between dilutors at different temperatures for above parameters after preservation. The study revealed that preservability of semen was better upto 72 hrs at 50C in EYC dilutor.