International Journal of Geomatics and Geosciences

Open Access
  • Year: 2015
  • Volume: 5
  • Issue: 4

Effect of Spacing and Refuge Crops on Gene Transfer of NptII Gene from Bt cotton to Soil Bacteria and on Seed Cotton Yield

  • Author:
  • Raghuvir Singh1, Vipin Prasad2, Sobita Simon3, Mercy Devasahayam1,
  • Total Page Count: 10
  • DOI:
  • Page Number: 687 to 696

1Centre for Transgenic Studies, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Naini, Allahabad-211007, Uttar Pradesh

2Department of Horticulture, Allahabad School of Agriculture, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Naini, Allahabad-211007, Uttar Pradesh

3Department of Plant Protection, Allahabad School of Agriculture, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Naini, Allahabad-211007, Uttar Pradesh

Abstract

The proportion of bacteria resistant to kanamycin from Bt and non Bt cotton (nBt) cotton crop soil at 0.65m spacing was higher than crop soil samples from 0.35m spacing. nptII specific PCR total bacteria DNA samples from Bt cotton soil were negative confirming the presence of indigenous kanamycin resistant soil bacteria. Bt seed cotton yield in presence of 50% refuge was higher when sown at spacings of 0.65m than at 0.35m. In presence of 50% refuge a 94% increase in Bt seed cotton yield was obtained. The presence of 50% nBt refuge increased the seed cotton yield of Bt cotton plants and; did not cause horizontal gene transfer of the nptII gene to soil bacteria.

Keywords

Bt cotton, horizontal gene transfer, kanamycin resistant soil bacteria, nptII, Seed cotton yield