Department of Horticulture, University of Hawaii, Honolulu, Hawaii
1Published with the approval of the Director of the Hawaii Agricultural Experiment Station as Technical Paper No. 931. This study was supported by the grants from the U.S. Atomic Energy Commission.
Comparative studies of the similarities and differences in the isoenzymic pattern of several enzymes in the extracts of root, coleoptile and the first leaf of seedlings of Chincse Spring without and with the umbellulata chromosome segment (in Transfer CI. 13296) were made. Horizontal gel electrophoresis techniques were employed to resolve and stain the isoenzymes. The differences in the isoenzymic pattern with respect to the presence or absence of onc or more isoenzymes of the enzyme systems studied were evaluated. The results show:
1. Presence of one additional slow moving anodal esterase isoenzyme (Esta-1a) in the extracts of the three tissues of Transfer seedlings and its absence in Chinese Spring.
2. Presence of an additional fast moving anodal esterase isoenzyme (Esta-15a) in the extract of roots of Chinese Spring seedlings and its abscnce in Transfer.
The presence and the absence of these isoenzymes have heen correlated with the presence of umbellulata chromosome segment in the first case and to the absence of the segment from chromosome 6B in the second case. I soenzyme variation between the tissues were observed and have been discussed in relation to development and differentiation.
