Mutation Frequency in Barley Following Treatment with EMS or Gamma Rays and Post-Treatment with GA

Ethyl methanesulphonate and gamma radiation treated seeds of barley were soaked in distilled water or 1000 PPM gibberellic acid for two hours to study the effect of the post-treatments on the chlorophyll mutation frequencies. Post-soaking in water resulted in reduction in mutation frequencies. Mutation frequencies following mutagen plus GA treatments were slightly more as compared to mutagen plus water treatments. Maximum mutation frequencies, however, were obtained when no post-treatment was given.