Division of Genetics, Indian Agricultural Research Institute, New Delhi
*Present address: Department of Botany, Marathwada Agricultural University, Parbhani (Maharashtra).
Online published on 25 January, 2012.
Five varieties of L. sativus were treated with γ-rays, EMS, NMU and the combination of γ-rays and NMU. The mutation frequency in M2 was dose-dependent. NMU was the most potent mutagen in inducing mutations. The combination treatment did not reveal synergistic effect. The ratio of chlorophyll to morphological mutations in M2 was 1⋅5 to 2 ⋅ 1. The varieties differed in radiosensitivity. With recurrent irradiation, the frequency of mutations declined indicating the diploid nature as well as acquisition of radioresistance.
