1Tissue Culture and Mutation Breeding Laboratory, Botany Department, Kakatiya University, Warangal, 506009
*Mulberry Breeding and Genetics Section, Central Sericultural Research and Training Institute, Srirampuram, Mysore, 570008.
Seeds of pink flowered (PF) and white flowered (WF) Catharanthus roseus were soaked in distilled water for 24 h and treated with gamma rays and 0.1% EMS separately and in combination. Six types of chlorophyll mutations, viz., xantha, albina, chlorina, viridis, maculata and tigrina were recovered to M2 generation of both forms. The frequency of chlorophyll mutations was found to be dependent on the dose, of gamma rays and duration of treatment with EMS. Higher frequency of chlorophyll mutations was noticed in PF, which is mutagenically more sensitive than WF. It was also noticed that the combination treatments of gamma rays and EMS enhanced the frequency of chlorophyll mutations.
Catharanthus roseus, chlorophyll mutations, gamma rays, ethyl methanesulphonate