Department of Plant Physiology and Biochemistry, Kazakh State University, Alma-Ata 480121, USSR.
* Present address: Plant Tissue Culture Laboratory, Department of Plant Breeding and Genetics, J.N. Agricultural University, Jabalpur 482004.
Anthers from two cultivars of barley (Hordeum vulgare L.), Chemigovski 5 and Oneprovski 435, were cultured on N6 liquid and semisolid agar media with three different levels of 2,4-D (2.0, 4.0 and 6.0 mg/litre) and 0.5 mg/litre kinetin. Significant results were obtained for and rogenic calli and embryoid production with increased 2,4-D level, especially in liquid medium preconditioned with isolated ear heads. As a result, number of plant lets was also high although not much difference was observed among media supplemented with three levels of 2,4-D for the regeneration of green plant lets. Increased 2,4-D produced more androgenic calli than embryoids. The plantlets regenerated were mostly dihaploids (2n = 2x = 14) and ranged from 65.7% to 84.6% for both the cultivars.
Hordeum Vulgare, 2,4-D, androgenic calli, embryoids, dihaploids
