Plant Biotechnology Centre, Department of Plant Breeding, Punjab Agricultural University, Ludhiana 141004.
Regeneration of haploids by tissue culture in groundnut would facilitate the rapid production of homozygous lines. Response of genotypes to different media was studied. Murashige and Skoog [1] medium supplemented with 3% sucrose or coconut milk, gave the best callus proliferation and rhizogenesis. Variable chromosome numbers, ranging from n=20 to n=40, were observed in the callus cells. The responding anthers revealed early segmentation of pollen, multicellular pollen and pollen embryo in all the three genotypes.
Arachis hypogaea L., anther culture, pollen embryogenesis
