Effect of synthetic detergents on interphase nuceli in Allium cepa L.

Three commonly used detergents, Sansar, Sunlight and Key, were evaluated for their comparative efficacy in blocking the various stages of interphase: G₁ and G₂ arrest of interphase nuclei after detergents treatment was recorded, which was found to be timeand concentration-dependent. However, no significant difference in the effects among three detergents was observed. The G₁- and G₂-arrest of cells is possibly due to nonsynthesis of proteins required for G₁-S transit and G₂-M transits, respectively. Further, at lower concentration of treatments, blockade in DNA synthesis at S phase was observed as indicated by reduction in G₂-cell populations.