Department of Agricultural Botany, Meerut University, Meerut 250005.
The nuclear DNA content was estimated in 13 varieties each of pigeonpea (Cajanus cajan (L.) Millsp.) and pea (Pisum sativum L.) using Fuelgen cytophotometry. Data were recorded on 25 2C and 4C nuclei in each accession with two replications. Significant differences were found among the varieties of the same species. DNA density (DNA content/nuclear area) increased with increase in DNA content. 4C nuclei exhibited higher density than 2C nuclei in each case. The 2C DNA values computed from 4Cnuclei were slightly lower than those estimated from 2C nuclei directly.
Pigeonpea, pea, intraspecific variation, nuclear DNA content
