Department of Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar 263145.
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Somaclonal variation was studied in SC2 generation of in vitro regenerated resistant plants of barley cv. Dissa. Two seedling mutants, internodeless and freak narrow leaf were obtained. Nucleus was multilobed in most of the cells in the somaclonal line which showed internodeless mutant. 50% of in vitro selected somaclonal lines showed stable transmission of resistance to Helminthosporium sativum.
Somaclonal variation, Helminthosporium sativum, Hordeum vulgare, nuclear abnormalities