The D genome encoded high molecular weight (HMW) subunits of glutenin 5 + 10 (Glu-1 score 4) from high grain-protein stock TW-1 and cv. Shera were transferred to cv. Kalyan Sona with allelic subunits 2 + 12 (Glu-1 score 2) using backcrosses. Selections in the BC2F5 and BC1F5 generations, homozygous for HMW subunits 5 + 10 or 2 + 12 were analysed for SDS sedimentation volume. Family means of selections carrying subunits 5 + 10 showed higher sedimentation volume than those with subunits 2 + 12. Thirteen selections were numerically superior to the recurrent parent Kalyan Sona in sedimentation volume, 12 of these carried subunits 5 + 10. These selections resembled Kalyan Sona in the A and B genomecoded HMW subunits of gluten in, grain protein content, grainyield and plant type. Transfer of desirable HMW subunits of glutenin for modifying Glu-1 score and quality of cultivars using backcrossing and SDS-PAGE of half grains is demonstrated.
Triticum aestiuum, wheat, glutenin, SDS-sedimentation test, SDS-polyacrylamide gel electrophoresis, quality
