Department of Agricultural Botany, Institute of Advanced Study Meerut University, Meerut 250 005.
* Present address: Department of Genetics and Plant Breeding, G. B. Pant University of Agriculture & Technology, Pantnagar 263145.
A study on the effect of genotype and culture medium on callus induced in seed explants of ten rice cultivars indicated significant variances due to genotypes, media and genotype x medium interactions (except callus induction frequency) for eight characters related to callusing and plant regeneration. Among the genotypes, Pusa Basmati 1 gave the best overall callusing response. For plantlet regeneration, variety Pant Dhan 4 recorded the highest number of green spots and shoots while Sarju 52 had the highest potential for the length of longest shoot and rooting. Likewise the MS medium supplemented with 2.0 mgl−1, 2,4-D for callusing and 2.0 mgl−1 IAA plus 3.0 mgl−1 KN (MS (3)) for shooting while 3.0 mgl−1 IAA plus 4.0 mgr 1 KN (MS (4)) for rooting gave the best results. Some of the selected combinations like Pant Ohan 4 on MS medium supplemented with 2.0 mgl−1 2,4-D plus 1.0 mgl−1 KN displayed excellent callusing capacity while Pant Ohan 4 on MS (3) and MS (4), Sarju 52 on MS (4) and Pusa Basmati 14.0 mgl−1 lAA plus 4.0 mgl−1 KN (MS (5)) exhibited best regeneration capacity, and therefore, of fer good scope for high totipotency using appropriate genotype and media with propercombination of hormones.
Rice, Oryza sativa L, callus growth, plant regeneration, tissue culture
