Effect of genotypes and nutrient media on immature embryo culture of wheat

Immature embryo of 14 genotypes of Triticum aestivum and 4 genotypes of T. durum were cultured on four modified MS medium supplemented with 30 g.l⁻¹ sucrose, 8 g.1⁻¹ agarand different level of growth hormones, viz. 2 mg.l⁻¹ 2,4-D; 5 mg.l⁻¹ 2,4-D; 2 mg.l⁻¹ 2, 4-D + 100 ml.l⁻¹ coconut milk; and 2 mg.l⁻¹ 2,4-D + 0.5 mg.l⁻¹ kinetin. Highly significant differences in the response of genotypes, culture media and genotype X medium interactions were observed for callus initiation from immature embryos, formation of embryogenic calli and plantlet regeneration.