Linkages between restriction fragment length, isozyme and morphological markers in rice

A genetic linkage map of rice comprising 130 centimorgans (cM) was constructed from ten restriction fragment length, six isozyme and one morphological markers in F₂ population of a rice cross (IR 36 X Ma Hae). The DNA probes were labelled through nonradioactive dUTP. The relative distance between two markers varied from 5.7 cM to 24.1 cM. The morphological marker (Xa-4A: bacterial leaf blight resistance) could be placed between restriction fragment length polymorphic (RFLP) probes RG 303 and and RZ 536 at the distance of 18.9 cM and 16.1 cM, respectively. The RFLP marker RG 167 and isozyme marker Pgd-1 were located at the same position, i.e. 5.7 map units away from RG 1094. None of the isozyme marker could be detected to be closely linked to morphological marker under study.