Department of Plant Breeding, G. B. Pant University of Agriculture and Technology, Pantnagar 263 145.
* Present address: Centre for DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012.
Electrophoretic variations were used to identify yellow mosaic resistant, tolerant and susceptible soybean lines at early stages of the plant growth. Five enzyme systems, i.e., peroxidase (Po), catalase (Cat), polyphenoloxidase (Ppo), esterase (Est) and ribonuclease-I (RNase) were studied. The Po bands numbers 5, 7, 8 and 12 in 6-day-old cotyledons; Cat 1, 2, 3 and 4 in 3, 6 and 12-day-old tissues; Ppo 6, 7, 9 in 3 and 6-day-old tissues; Est 1, 3, 5, 6 and 8 in 3 and 6-day-old seedlings and RNase 1, 2, 3, 4 and 5 in 3, 6 and 12-day-old tissues were the most prominent bands which can be used as biochemical markers for identification of resistant, tolerant and susceptible soybean lines at these specific stages.
Isozyme, soybean, electrophoretic analysis, peroxidase, catalase, esterase, ribonuclease-I
