Confirmation of digenic inheritance of cotyledon colour in lentil (Lens culinaris)

The F₃ seeds harvested from F₂ populations of five crosses between parents with orange and light green cotyledons were analysed. The F₃ segregation confirmed the earlier reported digenic control of cotyledon colour in lentil. Further, four new crosses were made between F₂ plants of different crosses which were raised from seeds with yellow and brown cotyledons. All F₁ seedshad orange cotyledons. The F₂ seeds harvested from F₁ plants raised in phytotron were analysed immediately for cotyledon colour. The F₂ seeds segregated in the ratio of 9 orange: 3 yellow: 3 brown: 1 green. The results from the new crosses also confirm digenic control of cotyledon colour in lentil.