Deptt. of Genetics and Plant Breeding, G. B. Pant University of Agriculture and Technology, Pantnagar 263145.
* Author for correspondence.
Treatments with mono-and bifunctional ethylated (NEU, NDEU) and methylated (NMU, NDMU) urea and ethylmethane sulphonate (EMS) in Manhar, an early maturing (120 days) variety of indica rice reduced the germination, seedling survival, pollen fertility and spikelet fertility percentage in M1 generation. NEU produced the highest frequency of viable mutations in the M2 generation, on the basis of segregating M2 progenies as well as mutants/100 M2 seedlings. Treatments with NEU (6 h) also produced the widest spectrum and maximum relative frequency of viable mutations in M2 generation. The relative mutation frequency of monofunctional compounds NEU and NMU was larger than their respective bifunctional compounds NDEU and NDMU. The most effective and efficient mutagens were 0.01% NMU and 0.05% NDEU, respectively.
Mutagen, mutations, effectiveness, efficiency, rice
