Gamma radiation and ems treatment of black cumin cultivars for mutational bioassays

Ten different types of chlorophyll mutations were induced in two cuItivars of black cumin (Nigella sativa L.) after gamma irradiation and EMS treatment. Mutation frequency was proportional to dose for gamma rays, but not for EMS. Higher doses of gamma rays and lower concentration/duration of EMS were most efficient. Difference in the response of both cultivars due to mutagenic treatment indicate variation in the genetic architecture of the two cultivars. Origin and use of black cumin cultivars for mutagen bioassays studies are discussed.