The Indian Journal of Genetics and Plant Breeding

SCOPUSWeb of Science
  • Year: 1997
  • Volume: 57
  • Issue: 4

Identification of Sesamum alatum x Sesamum indicum hybrid using protein, isozyme and rapd markers

  • Author:
  • M. Parani, K. N. Singh, Sree Rangasamy, R. S. Ramalingam
  • Total Page Count: 8
  • DOI:
  • Page Number: 381 to 388

School of Genetics, Tamil Nadu Agricultural University, Coimbatore 641 003.

*Present Address : M. S. Swaminathan Research Foundation, III Cross Road, Taramani Institutional Area, Chennai, India 600 113.

Abstract

With the objective of transferring phyllody disease resistance, hybridization has been done between Sesamum alatum and S. indicum. Identical chromosome number of the species, ambiguous morphological characters and lack of segregation in F2 necessitated to look for molecular markers to establish the hybridity. SDS-PAGE of seed protein revealed transfer of five male-specitic proteins to the putative hybrid. Further, inheritance of the est D locus of esterase, prx A and prx E loci of peroxidase and RAPDs of decamer random primers have not only establishing the hybridity but were also found to be useful as markers to distinguish the hybrids from the seIfed progenies.

Keywords

Sesamum alatum, Sesamum indicum, molecular markers