Identification of Sesamum alatum x Sesamum indicum hybrid using protein, isozyme and rapd markers

With the objective of transferring phyllody disease resistance, hybridization has been done between Sesamum alatum and S. indicum. Identical chromosome number of the species, ambiguous morphological characters and lack of segregation in F₂ necessitated to look for molecular markers to establish the hybridity. SDS-PAGE of seed protein revealed transfer of five male-specitic proteins to the putative hybrid. Further, inheritance of the est D locus of esterase, prx A and prx E loci of peroxidase and RAPDs of decamer random primers have not only establishing the hybridity but were also found to be useful as markers to distinguish the hybrids from the seIfed progenies.