Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012.
* Present address: Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat, Assam 785 013.
Studies on pollen germination, pollen tube growth and micropylar penetration in the cross D. tenuisiliqua x B. campestris revealed that 0.06% ovules were fertilized at 5 days after pollination. Field pollination did not set seeds in this cross. Sequential culture of ovaries and ovules helped obtain intergeneric hybrids. Starch gel electrophoresis for peroxidases established true nature of the hybrids.
Intergeneric hybrids, embryo rescue, Diplotaxis tenuisiliqua, Brassica campestris