Cytogenetics Laboratory, Department of Genetics, CCS Haryana Agricultural University, Hisar 125 004.
* Present address: Department of Biocybemetics, Institute of Nuclear Medicine & Allied Sciences, Lucknow Marg, Delhi 110 054. Fax: 291–95059 Email; sudhir@drinma.ren.nic.in.
A laboratory mixture of two insecticides, viz., carbaryl and lindane (1:1) was tested for its clastogenicity in the bone marrow erythrocytes of swiss albino mice. The insecticidal mixture was injected intraperitoneally at three dose levels as a suspension in 1% gum acacia, and chromosome aberrations assay and micronucleus test were performed in both sexes separately. Ethylmethanesulphonate (EMS) was taken as positive reference mutagen. An increase in the frequency of micronucleated erythrocytes was observed following treatment with the test chemical mixture. In chromosome aberrations assay, a significant induction was observed at 185 mg/kg bw. at 24h post-treatment time. The female mice appear more tolerant to the test mixture than the male mice.
Clastogenicity, lindane, carbaryl, mice, micronuclei, chromosome aberrations, erythrocytes
