School of Genetics, Tamil Nadu Agricultural University, Coimbatore 641 003.
* Present address: Division of Crop Improvement, Sugarcane Breeding Institute, Coimbatore 641 007.
Callus cultures derived from mature embryos and inflorescence explants of guinea grass [cv. GG2), Panicum maximum J. at different stages of development were used to study the patterns of iso esterases and iso acidphosphatse, Isozyme patterns were found to vary with all developmental stages studied. Twelve isoesterases were detected in the embryogenic calli. With the germination of embryoids into plantlets, only two of these isozymes were retained. Acid phosphatase was found to be organ and tissue specific. The novel isozymes that appeared in the embryogenic and organogenic calli can be used as marker to distinguish between embryogenic and non-embryogenic calli.
Guinea grass, acid phosphatase, esterase, isozyme marker, embryogenic calli
