Laboratory of Molecular Biology & Biotechnology, Orissa University of Agriculture and Technology, Bhubaneswar 751 003.
* Directorate of Rice Research (ICAR), Hyderabad 500 030.
SDS-PAGE of total seed proteins of 11 rice genotypes resistant to green leafhopper (GLH) and a susceptible variety, Taichung (Native) T(N) 1 facilitated scoring of 11 distinct polypeptide bands of heterogeneous molecular weights ranging from 84.1 to 24.0 kD. Seven bands exhibited polymorphism with regard to presence/absence or molecular weight. Two bands (46.8 and 42.7 kD) were present only in the susceptible variety and are potentially reliable markers in screening for GLH resistance. Five bands of 53.7, 38.0, 33.9, 28.2 and 24.0 kD were polymorphic for presence/absence or molecular weight even within the resistant genotypes. These polymorphic bands can be used as markers in breeding for GLH resistance as well as for detection of identification of unknown resistance genes and understanding the genetics of resistance.
Rice, Green Leafhopper, Resistance, Protein Markers, Marker Assisted Breeding
