The Indian Journal of Genetics and Plant Breeding

SCOPUSWeb of Science
  • Year: 2002
  • Volume: 62
  • Issue: 1

Inheritance and linkage relationship between morphological and RAPD markers in lentil (Lens culinaris Medik.)

  • Author:
  • M. E. Hoque1, S. K. Mishra, A. Sarker2
  • Total Page Count: 6
  • DOI:
  • Page Number: 5 to 10

Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012.

Present address:1 Plant Breeding Division, BARI, Gazipur 1701, Bangladesh.

Abstract

Inheritance and linkage relationship befween eight morphological and RAPD markers were studied in lentil based on the analysis of F2 pdpulatlon of the crossL 6163 x L 830. All morphological traits showed monogenip mode of inheritance. Plant segregation analysis between morphologicalal markers revealed that the traits leaf, stem and pod igmentation were linked to each other. One hundred and fourty RAPD decamer primers were screened for detection of polymorphism in the two parents viz., L 6163 and L 830. Only nine primers showed reproducible polymorphism among the parents. Out of nine polymorphic primers only two primet:(OPB 0260c and OPM 06900) gave expected monogenic mode of inheritance. Joint segregation analysis between RAPD marker 06900 and other morphplogical markers revealed that the traits leaf and stem pigmentation are linked with marker OPM 60900 at the map distance of 7.23 and 12.50 Kosambi unit, respectively. The other marker OPB 02600 did not show any linkage among the morphological traits under study.

Keywords

Lens culinaris, linkage, morphological markers, RAPD