National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012.
1Present address: Central Potato Research Institute, Shimla 171 001.
Mesorhizobium ciceri strain MC18-7, nodulating chickpea was used to generate nod-mutants to study primary nodulation responses. Transposon insertion was carried out in pRmSL-26 using in vitro transposition by EZ:: TN <KAN 2> insertion system. Transposon insertion sites in nod ABC and nod D genes in pRmSL-26 were used for marker exchanging in M. ciecri strain MC 18-7. In total three mutants, one nod ABC−mutant and two nod D−mutants were obtained. The common nod ABC−mutant showed complete lack of root hair infection/deformation, while nod D−mutants showed delayed/reduced primary modulation responses.
Chickpea, Mesorhizobium ciceri, nodulation genes, in vitro transposition, marker exchanging
