Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad 580 005.
Twelve cotton genotypes representing different species and adapted to different agroclimatic conditions have been screened for in vitro regeneration. Diploid species G. herbaceum and G. arboreum gave higher response for in vitro dedifferentiation than tetraploids, G. hirsutum (4X) and G. barbadance (4X). Hypocotyl explant showed higher response than cotyledons for callus induction. Rooting was observed in media containing auxins to cytokinins in a 20:1 ratio in most genotypes. This study finally emphasized the screening large number of genotypes for regeneration and to study genetics of regeneration using Coker 312, the only regenerable genotype.
Cotton, differentiation, regeneration
