The Indian Journal of Genetics and Plant Breeding
SCOPUSWeb of Science
  • Year: 2003
  • Volume: 63
  • Issue: 3

Screening of wheat varieties for use as donors for production of near isogenic lines with different seed protein alleles

  • Author:
  • M. Rathore, D. P. Mishra1, N. K. Singh2
  • Total Page Count: 3
  • Page Number: 206 to 208

Department of Molecular Biology and Genetic Engineering, GBPUA&T, Pantnagar 263 145.

1 Department of Biochemistry, CBSH, GBPUA&T, Pantnagar 263 145.

Abstract

Sequential extraction of water-soluble albumins, salt-soluble globulins (triticin), alcohol-soluble gliadins and acid-soluble glutenins, the four major classes of wheat seed storage proteins, was done from 31 wheat cultivars. SDS-PAGE of the protein fractions showed high variation in the banding pattern. Varieties with different alleles in comparison to the recurrent parent HD 2329 were chosen for all the four fractions of seed storage proteins. A total of 12 cultivars were finally chosen to make NILs for forty different seed storage protein alleles in HD 2329.

Keywords

Wheat, seed storage proteins, allelic variation, SDS-PAGE, NILs