Department of Genetics and Plant Breeding, College of Agriculture, G.B.P.U.A.&T., Pantnagar 263 145.
The seed proteins of 24 varieties/advanced breeding lines of Indian bread wheat were fractionated using Sodium Oodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to determinbe their high Mr glutenin subunit composition. Amongst the 24 varieties/advanced breeding lines, three alleles were identified for the Glu-A 1 locus (subunits 1,2* and null phenotype), three alleles for the Glu-B1 locus (7+8, 7+9 and 17+18) and two for the Glu-D1 locus (2+12 and 5+10). These subunits showed high polymorphism. Based on these subunits 9 different groups were observed in 24 genotypes. Two genotypes viz., UP 2441 and CPAN 3004 had distinct group from the remaining genotypes. The genotype UP 2441 showed null, 17+18 and 2+12 and CPAN 3004 revealed 1, 7+9 and 2+12 subunits. This suggested th'e usefulness of seed protein electrophoresis showing high Mr glutenin subunits in identifying and characterizing the different wheat genotypes.
Bread wheat, seed protein, electrophoresis, HMW subunit of glutenin
