High molecular weight glutenin subunits variation biscuit making quality in wheat (Triticum aestivum Theil.)

The SDS-PAGE of 30 wheat genotypes demonstrated the presence of 1, 2* and null HMW-GS at Glu-1A locus, 7+8, 7+9, 14+15 and 17+18 at Glu-1B and 2+12 and 5+10 HMW-GS at Glu-10 locus. The two durum wheats POW 215 and WH 896 lacked all the HMW-glutenin subunits, viz., 1, 2*, null, 7+8, 7+9, 14+15, 17+18, 2+12 and 5+10. The 30 wheat genotypes displayed polymorphism for HMW-GS at Glu-1A, Glu-1B and Glu-1D loci. The diversity of the Genotypes for HMW-GS was also apparent from the dendrogram drawn using UPGMA cluster analysis. The wheat genotypes Raj 3765, UP 2576 and UP 2530 having cookie spread ratio of 6.1, 7.0 and 10.3, respectively carried 2+12 subunits at Glu-1D suggesting a relationship between better biscuit making quality and HMW-GS 2+12. HMW-GS 5+10 was shown to be associated with inferior biscuit making quality in PBW 175, PBW 343, UP 2567, UP 2569, UP 2425, UP 2562 and PBW 373. However, this relationship did not hold true in few cases possibly due to influence of other factors.