Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri 413 722.
Molecular characterization of the mitochondrial DNA [mtDNA] from the etiolated pearl millet [Pennisetum glsucum (L.) R. Br.] seedlings of cytoplasmic male sterile (CMS) line having A1 cytoplasm alongwith Its maintainer, restorer and restored lines of hybrid RHRBH-8609 (Shradha) revealed polymorphism by 10 RAPD primers. Only four primers showed polymorphism between a CMS, RHRB-1A and Its fertile Isonuclear line, RHRB-1B. However, 5 primers showed polymorphism between the CMS line and its restorer line, RHRB-138R. The primers OPC15, OPC16 and OPG13 generated fragments of ∼3.90 kb, 1.59 kb and 1.02 kb size only In the mtDNA of a male sterile line which might be associated with the CMS phenotype of RHRB-1A in pearl millet.
Pearl millet, cytoplasmic male sterility, mtDNA, RAPD markers
