High frequency plant regeneration from an amphiploid hybrid seed of Glycine max x G. tomentella through tissue culture and hybridity testing of the regenerants

Fifty-five plants from the amphiploid hybrid (2n = 118) of Glycine max (2n = 40) and tomentella (2n = 78) were regenerated from a single seed in tissue culture. The embryonic axis converted into a solid, green mass and gave rise to shoot buds on 6-benzyl aminopurine (BA)supplemented medium. Shoots were eventually obtained on the same medium. Rooting occurred in 100% of the plants on Whites medium containing coumarin (9 mgl⁻¹). A total of 50 plants survived the hardening treatment. All of the tissue culture-raised plants were morphologically similar and flowered under greenhouse conditions. The hybridity of the regenerated plants was confirmed by using morphological and simple sequence repeat (SSR) markers. Attempts are being made to cross these plants with soybean to generate backcross-derived lines for further genetic studies.